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828 Uppsatser om Sample preparation - Sida 1 av 56

XRF-analys av förorenad mark : undersökning av felkällor och lämplig provbearbetning

X-ray fluorescence (XRF) detection of heavy metals is a cost- and time-effective method for investigation of polluted areas. Compared to laboratory analysis, XRF analysis is limited by high detection limits and uncertainties in some situations. Preparation of samples is known to affect the results of measurements.The purpose of this thesis is to bring a deeper understanding of how different factors affect the results of XRF-analysis. A large number of measurements have been made with the instruments Niton XLt 700 and Niton Xli 700. Results from measurements of lead, zinc and copper have been analysed.This study has shown that a greater moisture content will give a lower measured concentration for the same sample.

Evaluation of Different Extraction- and Analysis Methods for Calprotectin in Feces

Background Calprotectin is a protein expressed in the cytoplasm inside the neutrophile granulocytes. During inflammatory bowel disease (IBD), the neutrophile granulocytes are involved in a complex interaction at the inflammatory area where they die and release their content into the intestinal lumen. Therefore, calprotectin in stool is a suitable marker for diagnosis and measurement of the disease-activity in patients with IBD. The most commonly used method to detect calprotectin in stool is ELISA, but the process of manual preparation of stool samples is time-consuming.Aim The objective of the study was to evaluate an extraction method that could replace manual preparation of fecal samples and to compare different methods for measuring Calprotectin in stool using two ELISA-methods from two manufacturers and one rapidtest.Methods For extraction of calprotectin from stool samples we used sample collector tubes from Epitope Diagnostics and fecal preparation kits from Roche. Two different ELISA-kits for measuring calprotectin concentration in stool were compared.

Frosttolerans hos douglasgran av olika ursprung och tillväxttyp

This report evaluates whether normal approximation or resampling is to prefer for estimating the distribution of the sample mean and functions of the sample mean. The evaluation relies on simulation studies. The observations of the sample are allowed to be differently distributed. In the case of sample means they are also allowed to be dependent.For sample means the two approximations behaves very similar. The most important component whether we have a good or a bad approximation is how good the approximations catch the variance of the true distribution.

TaqMan® Sample-to-SNP Kit? : evaluation of kit for low-cost and fast preparing of DNA-samples before genotype analysis

 Genotyping can be used to link genetic variation among individuals to certain diseases or conditions. Some known disorders and states that are dependent on single nucleotide polymorphism (SNPs) are lactose intolerance, venous thrombosis, hereditary hemochromatosis and the difference in sensibility among people to metabolise drugs.In this project a new kit, TaqManÒ Sample-to-SNP KitÔ for extraction of DNA and preparation of the extract for genotyping with real-time PCR and allelic discrimination, was evaluated. QIAamp® DNA Blood Biorobot® MDx Kit was used as the reference method.The purpose of the comparison was to find a method that makes DNA extraction from blood samples cheaper and faster, but with the same reliability as the reference procedure.The results of the evaluation showed a complete agreement of the genotype results between the methods tested, which means that the new method was as reliable as the reference method. The costs of reagents and material would be reduced with 52% if the new method is adopted, that alone would result in a cost reduction of 144 000SEK a year with a sample volume of 650 samples/month. The time for DNA extraction would also be reduced with the new procedure. .

Statistisk analys av barnpsykiatrisk symptomatologi som ökar risken för senare personlighetsstörning

This report evaluates whether normal approximation or resampling is to prefer for estimating the distribution of the sample mean and functions of the sample mean. The evaluation relies on simulation studies. The observations of the sample are allowed to be differently distributed. In the case of sample means they are also allowed to be dependent.For sample means the two approximations behaves very similar. The most important component whether we have a good or a bad approximation is how good the approximations catch the variance of the true distribution.

En jämförelse av stickprovsmetoder vid mätning av radioaktivetet på en yta

This study compares the sample variance of the mean in a simple random sample (SRS) and a systematic sample from a surface. The study also compares the prediction error variance in a SRS and systematic sample from a random field. Recommendations are presented on which method to use in the clearance and exemption process in the nuclear industry. The results indicate that quadratic systematic sampling outperformed SRS in most instances and proved to be especially efficient in the presence of a long-ranged covariance function and high sampling intensity (i.e. short distances between observations).

En utvärdering av två olika sa?tt att skatta fördelningen till stickprovsmedelvärden från olikfördelade data - normalapproximation kontra resampling

This report evaluates whether normal approximation or resampling is to prefer for estimating the distribution of the sample mean and functions of the sample mean. The evaluation relies on simulation studies. The observations of the sample are allowed to be differently distributed. In the case of sample means they are also allowed to be dependent.For sample means the two approximations behaves very similar. The most important component whether we have a good or a bad approximation is how good the approximations catch the variance of the true distribution.

De bortglömda barnen : En kvalitativ studie om hur några lärare arbetar med de nyanlända eleverna i den svenska skolan och om de olika undervisningsformerna bidrar till integration

Children of school age, who have just moved to Sweden can either start in a preparation class or in a normal class. There is no Swedish law or national policy that determines how new children will start and continue their education in Swedish schools. The decision is up to the schools or the communes.This study investigates what kind of educational methods teachers, from a sample of two different schools, are using to educate the newly-arrived students and if these methods are making any contribution to integrating these children into the Swedish school system. The study will raise questions like, how are the new students introduced to the Swedish school, what is the purpose of having the children in preparation class as opposed to normal class, and what methods do the interviewed teachers use to integrate the new children into the school system?Interviews were conducted with four teachers, an associate professor and a senior lecturer in education, who has done several years of research about children from abroad coming in to the Swedish school system.

Präststudenternas och verksamma prästers uppfattning om språkliga förberedelser inför sin predikogärning

This essay was written in order to elucidate how the priests-to-be and working priests experience the preparation for preaching during their training and if their identity as preachers depends on their preaching; how they refer to rhetoric and what the preparation training looks like.There is no Swedish research within this field, and even the existing international studies concentrate mostly on writing preparation within other occupations, e.g. economics, health care and public sectors. These studies also show that there are several discrepancies between the university preparation for professional writing and speaking and the professional discourse.In order to carry out the investigation the author has interviewed priests-to-be and working priests and examined the syllabi for homiletics and rhetoric. The result of this analysis offers a better insight into the nature of the training in preparing sermons that Swedish priests obtain during their professional education. The author refers to the results of other scientific investigations concerning preparation for professional writing and speaking and concludes that there are similar difficulties within the priest training, e.g.

Sjuksköterskans roll och upplevelser vid förberedelser av barn inför perifer venkateter sättning och blodprovstagning : En intervjustudie

The purpose of the study was to describe the nurse´s role and experiences aroundpreparation of children in front of peripheral venous catheter (PVC) placement andblood draw. The study used a descriptive design with qualitative approach and wasperformed on a pediatric clinic in Sweden. Semi-structured interviews were performedwith seven nurses of whom the first was a pilot interview, which was included in theresult. The recruitment of nurses was made by a purposeful sampling, maximumvariation sampling, with help of the quality coordinator of the pediatric clinic. Thecollected data was analyzed with the manifest qualitative content method.

Ren hud : En litteraturstudie om hur omvårdnadsåtgärden hudförberedelse har betydelse för postoperativa sårinfektioner

Postoperative site infections are the second most common health care associated infection in Sweden. Surgical procedures can lead to postoperative site infection causing great suffer for the patient and an extended care time with an increased cost as a result. In preparation for surgery, it is important that the skin is well prepared with antiseptics to decrease the risk of postoperative site infection. The purpose with this study was to shed light upon the skin preparation before surgery and its significance to postoperative site infection. Ten scientific articles have been scrutinized and compiled and two themes have been identified ? the preoperative and the intraoperative skin preparation.

Framtagande av formskal till luftberedningsenhet

SMC Pneumatics is a world leading company in pneumatics. In Sweden, they have about one third of the pneumatic market. The company has noticed that their air preparation series not always have the appearance that the costumers are looking for. Their competitors have on their similar products put more effort in making them more attractive. To solve this problem it has in this project been developed a concept for aForm Shell.

Jag är ny här - får jag vara med? : En studie av nyanlända elevers sociala integration i skolan

The aim of the study was to evaluate the social integration amongst newly arrived students in the two different school systems Sweden welcomes them into. The first school system takes in newly arrived students into preparation classes whilst the other integrates the students directly into ordinary classrooms. The examination is based on qualitative observations in the classroom, canteen, and playground, and interviews with teachers and a select few students from each school system.The result of the study showed that newly arrived students in ordinary classes are socially integrated more into the school than the newly arrived students that are welcomed into a preparation class. Newly arrived students are offered a larger chance of being integrated into the school environment if they are introduced directly into an ordinary class. In preparation classes, social integration is narrowed down to between the classmates.

Evaluation of preanalytic methods in order to shorten the processing time before identification of fungal microorganisms by the MALDI-TOF MS

Identification of fungi is based on macroscopic observations of morphology and microscopic characteristics. These conventional methods are time-consuming and requires expert knowledge. For the past years Matrix-assisted laser desorption ionization-time of flight mass spectrometry has been used for routine bacterial identification in clinical laboratories but not yet in the same extension for fungi. In this study three preanalytic preparation methods for fungi were evaluated in order to shorten the processing time in routine laboratory performance.Clinically relevant strains (n=18) of molds and dermatophytes were cultivated on agar plates and prepared according to the different preparation methods for protein extraction. Each strain was analyzed in quadruplicate by the MALDI Biotyper and the database Filamentous Fungi Library 1.0.The results showed that the genus and species identification rates of the least time-consuming direct extraction method were 33% and 11% respectively.

Lan File Share : Ett fildelningssystem åt Försvarsmakten

The Swedish Armed Force has a need to streamline the preparation of an exercise in tactical training for soldiers because the preparation of an exercise is currently laborious and time consuming when commanders manually share files to each computer that will be included in the exercise. The degree project was to create a file sharing program within the internal network of the Defence Forces? international centre (Swedint). The program is used by the training management to effectively start up an exercise where soldiers train tactical training in a simulated combat environment. The training management use this program to upload files to a server which notifies all the computers on the network to download these files.

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